Cortical and zona reactions of heat-activated mouse eggs.
نویسندگان
چکیده
Oocytes collected from superovulated Swiss albino mice were activated by heat-shock and/or fertilized in vitro. Electron microscopy showed that the cortical reaction in heat-treated eggs was incomplete. Digestion of the zona pellucida of untreated, heat-treated and fertilized eggs by pronase showed that zona pellucida hardening did occur but was weaker in heat-treated than in fertilized eggs. Fertilizability of heat-treated eggs decreased 1.5 h after heating. We concluded that, in heat-activated eggs, changes occur at the zona and plasmalemma but they are not identical with those in fertilized eggs.
منابع مشابه
Egg cortical granule N-acetylglucosaminidase is required for the mouse zona block to polyspermy
The mammalian egg must be fertilized by only one sperm to prevent polyploidy. In most mammals studied to date, the primary block to polyspermy occurs at the zona pellucida, the mammalian egg coat, after exocytosis of the contents of the cortical granules into the perivitelline space. The exudate acts on the zona, causing it to lose its ability to bind sperm and to be penetrated by sperm previou...
متن کاملI-13 Infertility with Impaired Zona Pellucida Adhesion of Spermatozoa from Mice LackingTauCstF-64
Background: Fertilization is a multistep process requiring spermatozoa with unique cellular structures and numerous germ cell-specific molecules that function in the various steps. In the highly coordinated process of male germ cell development, RNA splicing and polyadenylation help regulate gene expression to ensure formation of functional spermatozoa. Male germ cells express tauCstF-64 (Cstf2...
متن کاملHeat sensitivity of the cortical granule protease from hamster eggs.
Previous studies (Gwatkin, Williams, Hartmann & Kniazuk, 1973) showed that the zona reaction of the hamster ovum is produced by a cortical granule protease, which inactivates the species-specific receptor-for-spermatozoa located in the zona pellucida. This receptor was earlier shown to be sensitive to low concentrations of pancreatic trypsin (Hartmann & Gwatkin, 1971). Several years ago Austin ...
متن کاملSperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis.
At fertilization, spermatozoa bind to the zona pellucida (ZP1, ZP2, ZP3) surrounding ovulated mouse eggs, undergo acrosome exocytosis and penetrate the zona matrix before gamete fusion. Following fertilization, ZP2 is proteolytically cleaved and sperm no longer bind to embryos. We assessed Acr3-EGFP sperm binding to wild-type and huZP2 rescue eggs in which human ZP2 replaces mouse ZP2 but remai...
متن کاملThe zona reaction of hamster and mouse eggs: production in vitro by a trypsin-like protease from cortical granules.
The cortical granules of hamster ova were ruptured in vitro by electrical stimulation (standard treatment was 150 V, 1\m=.\0msec). Approximately 8 min were required for the cortical granule material so released to induce a complete zona reaction. The active material from the cortical granules was released into the medium from vitelli and was shown to act directly on the zona pellucida and on mo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of reproduction and fertility
دوره 73 2 شماره
صفحات -
تاریخ انتشار 1985